Reference Sequence of Chromosome 3B
Accueil
Projects
Milestone Publications
Reference Sequence of Chromosome 3B

Reference Sequence of Chromosome 3B

The first reference sequence for the largest wheat chromosome, 3B, was published in Science in July 2014 - in the same special issue were the draft sequence was published. This major achievement established a proof of concept and a template for sequencing the remaining chromosomes.

Reference

Choulet et a. (2014). Structural and functional partitioning of bread wheat chromosome 3B. Science, 345(6194), 1249721. https://doi.org/10.1126/science.1249721

Abstract

We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.

Modification date: 16 August 2023 | Publication date: 28 July 2021 | By: ic