Outline

More than 100 scientists from around the world attended the IWGSC scientific workshop held in Yokohama, Japan, on 8 September 2013. During the workshop, speakers highlighted the latest results from IWGSC projects. Kellye Eversole, IWGSC Executive Director, chaired the workshop. Simen Sandve, from the Norwegian University of Life Science presented the results from the Norwegian-BGI project to physical map and sequence bread wheat chromosome 7B. The Canadian contribution to the IWGSC, i.e., sequencing bread wheat chromosome 1A, was discussed by Andrew Sharpe from the National Research Council of Canada. Song Weining, Northwest A&F University in China, presented results from the recently completed physical map of chromosome arm 7DL and discussed the use of the map for reference sequencing and positional cloning. Dina Raats, from Haifa University in Israel, was selected as the Early Career Speaker for this workshop. She provided an overview of the gene space organization and evolution of chromosome arm 1BS gleaned through the completion of the 1BS physical map. Matthew Clarke, from The Genome Analysis Centre (TGAC) in the United Kingdom, gave an update of activities at TGAC. Marcelo Helguera from the Instituto Nacional de Tecnologia Agropecuaria in Argentina presented a report on the survey sequencing of bread wheat chromosome 4D. Kuldeep Singh, Punjab Agricultural University in India, gave an update on the rapid progress being made on the 2A physical map as well as the survey sequencing of 2A with 454 technology. Progress on the physical mapping of chromosome 5A was presented by Luigi Cattivelli from the CRA-Genomic Research Centre in Italy. Ekaterina Sergeeva, Institute of Cytology and Genetics in the Russian Federation, discussed the various stages they were going through in the development of the physical map of chromosome 5B. Abraham Korol, University of Haifa in Israel, described the advantages of using LTC software for physical mapping, contig assembly, and MTP selection, the gold standard for completion of IWGSC physical maps. Michael Alaux, Unité de Recherche Génomique Info (URGI) at the Institut National de la Recherche Agronomique (INRA) in France, discussed the genomic resources available at the IWGSC Sequence Repository hosted by the URGI.

The workshop concluded with a new "flash and dash" reporting session that included significantly shorter presentations. Hollie Webster, Murdoch University, led off with a report on the physical mapping and sequencing activities in Australia related to chromosome 7A. Viviana Echenique, CONICET in Argentina, discussed her project to analyze repetitive elements in bread wheat. The workshop concluded with an open forum for all participants to provide input into the IWGSC. During the discussion, there was a consensus that the IWGSC try to develop training webinars that would explain in detail how to utilize the resources being developed under the IWGSC umbrella.

Program

1:00 p.m. Eversole, Kellye. International Wheat Genome Sequencing Consortium. Welcome and introduction

1:20 p.m. Sandve, Simen. Norwegian University of Life Science & Hamar University College. Bread wheat chromosome 7B: genome assembly and transcript mapping. Slides

1:40 p.m. Sharpe, Andrew. NRC-PB. Reference sequencing of bread wheat chromosome 1A

2:00 p.m. Weining, Song. Northwest A&F University. Physical mapping of the wheat chromosome arm 7D: a foundation for reference sequence assembly and position-cloning

2:20 p.m. Early Career Speaker: Dina Raats. University of Haifa. The Physical Map of Wheat Chromosome 1BS Provides an Insight into its Gene Space Organization and Evolution

2:40 p.m. Clark, Matthew. The Genome Analysis Centre (TGAC). Wheat genome sequencing: an update from TGAC. Slides

3:00 p.m. Helguera, Marcelo. INTA. Wheat chromosome 4D survey sequencing: current progress towards the understanding of its structural organization. Slides

3:20 p.m. Singh, Kuldeep. Punjab Agricultural University. Physical Map and Survey Sequence of Chromosome 2A of Wheat. Slides

3:40 p.m. Break

4:00 p.m. Cattivelli, Luigi. CRA-Genomic Research Centre. Progress on physical mapping of wheat chromosome 5A. Slides

4:20 p.m. Sergeeva, Ekaterina. Institute of Cytology and Genetics. The stages of physical mapping of Triticum aestivum cv. Chinese spring chromosome 5B. Slides

4:40 p.m. Korol, Abraham. University of Haifa. LTC software for physical mapping: contig assembly, MTP selection and verification of clone overlaps at sequence level. Slides

5:00 p.m. Alaux, Michael. URGI (Unité de Recherche Génomique-Info), INRA, Versailles. IWGSC Sequence Repository. Slides

5:30 p.m. "Flash and Dash" reporting session.

• Webster, Hollie, Murdoch University. Physical map/BAC sequencing of chromosome 7A. Slides

• Echenique, Viviana, CONICET. Analysis and characterization of the repetitive sequences of T. aestivum chromosome 4D. Slides

Open Forum